Q30 bases什麼意思

"Q30 bases" is a term commonly used in the context of next-generation sequencing (NGS), also known as high-throughput sequencing. In this context, "Q30" refers to the Phred quality score, which is a measure of the accuracy of the base calls in the sequencing data. A Phred quality score of 30 corresponds to an error rate of approximately 1 in 1,000 bases, meaning that the sequencer is 99.9% confident in the accuracy of the base call.

The term "bases" refers to the individual nucleotides (A, T, C, or G) that make up the DNA sequence. In NGS, millions or billions of bases are sequenced, and each base call is assigned a quality score. The Q30 bases are the number of bases with a Phred quality score of at least 30.

In summary, "Q30 bases" is a measure of the quality of the sequencing data, indicating the number of bases that are called with a high degree of confidence (error rate of 1 in 1,000).